Comparative immunohistochemistry of Gut-associated lymphoid tissue (GALT) of, Huso huso in two warm and cold seasons

Abstract The main objective of recent study was comparative immunohistochemistry of Gut-associated lymphoid tissue (GALT) of, Huso huso in two warm and cold seasons. Ten specimens were collected from sturgeon breeding centers and after anesthesia, biometric was taken on them. 0.5 centimeter samples of primary, middle and end of intestine were removed and immediately were placed in Bouin solution. Rutine procedure of tissue prepration was done and sections in the thicknesses of 4-6 microns were prepared. Eventually they were taken under staining. Finally was performed general Hematoxylin-Eosin staining (H&E), histochemical staining of Periodic Acid Schiff (PAS), and (TUNEL) immunohistochemistry. Histomorphology and histometric results showed that the intestine consists of mucous, submucosa, muscularis and serousa. Comparative study of Gut-associated lymphoid tissue in two seasons was found that their density and composition are different. In cold season, the density of lymphoid tissue in base of the epithelium was more than the warm season.The number of lymphocyte cells in the mucosa and submucosa of different parts of the intestine in warm season showed a significant decrease compare to the cold season (p≤0.05). Also, the number of lymphocyte cells in the end of the intestine showed a significant increase during the cold season compare to the warm season (p≤0.05). In general, it has been shown that in immunohistochemical studies, the number of cells with apoptosis, especially in the warm season, were higher in all parts of the intestine than to the cold season (p≥0.05). Therefore, due to stimulation immune system in this part of the gastrointestinal tract, it can be considered to determine for vaccination time.